Effect of the Carbon Source and Plant Growth Regulators (PGRs) in the Induction and Maintenance of an In Vitro Callus Culture of Taraxacum officinale (L) Weber Ex F.H. Wigg

Taraxacum officinale (L.) Weber ex F.H. Wigg, commonly known as dandelion, is a cosmopolitan and perennial weed, which has medicinal properties. In vitro propagation methods are widely used on plants that have difficulties in cultivation and, consequently, low extraction yields of active metabolites. Thus, callus culture been considered to be useful for the accumulation several this study, we aimed establish an efficient protocol induction maintenance T. officinale, explant type, carbon source, light conditions, nine different combinations plant growth regulators (PGRs), such 1-naphthaleneacetic acid (NAA) (from 0.05 0.5 mg/L) 6-benzylaminopurine (BAP) 3.0 mg/L), were evaluated. The results showed hypocotyls roots from sterile seedlings best sources induction, with 100% callogenesis at every condition tested, more than 95% viability friability. Complete darkness medium supplemented sucrose 2.3% (w/v) mg/L NAA BAP conditions showing organogenesis high This study provides basis future studies improving large-scale further establishment suspension systems commercial purposes.